G-force is a function of acceleration.
Gravity works on a mass to pull it toward another mass. Large masses have higher levels of gravitational attraction. The force of gravity on Jupiter and Saturn is stronger than that on Earth. On the Moon, it is less than on Earth.
On Earth, gravity is a force that continues to pull us down toward the center of the Earth. The physical surface stops that acceleration. Our weight is the measure of that force acting on our mass.
Acceleration is a change in speed. When coasting (no acceleration nor deceleration forces) then there is no g-load (weightlessness in space).
Accelerating in a car, plane or spaceship causes g-loads. Again, it is the acceleration that is causing the load. Banking an airplane in a 60 degree bank will cause g-loads on the body due to centripetal force. Looping an airplane will do the same. An inside look causes positive g-load while and outside loop causes negative g-load. Both are measured by effect on the body. When upright, positive g-loads causing blood to flow out of the head toward the feet and negative g-loads causing blood to flow from feet to the head. Human bodies tolerate positive g-loads better than negative. Lying down, like in many fighter jets, helps mitigate the impacts as more of the body is level.
So toleration of space travel is a combination of tolerating g-loads during accelerating and deceleration phases and weightlessness (absence of acceleration) periods which tend to affect muscles, bone densities, etc.